FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15744586
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC269320.17105562508915764No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG226860.1440876247873396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA58750.017.0323267
ACACTAT66450.015.2753498
CACTATA72100.014.3778619
CCCCTTA43350.011.4725074
CAAACAC114500.09.8084095
AAACACT107750.09.487236
ACCAAAC114750.09.2977783
CCAAACA122100.08.9727644
TACCCCT63300.07.97225242
GTACCCC64700.07.8236571
ACCCCTT80950.07.1243183
TACCAAA182350.06.1674492
ATACCAA202200.05.6505661
TATTTAG100600.05.2954249
Produced by FastQC (version 0.11.5)