FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15572838
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG85100.019.9608759
GTGTACG56250.011.7743718
CTCGTGT40850.08.1069295
CTGTACG107400.07.77546078
CGTGTAC56700.06.98316147
AGTGTAC51350.06.72935157
TAGTGTA45600.06.63093956
GTGCGTT55500.06.48617273
GTAGTGT57150.06.04666045
TACATCT180200.05.67238331
TACGTGT53400.05.50838471
TAAATCG85000.05.45038461
TTGTACG63750.05.4204278
TACGGAC74100.05.3624910-14
TACTTAG61800.05.3546321
TACATTG145200.05.31775331
TACATAA151700.05.18685051
TACTTCA209800.05.0823911
GCGTTGT82200.05.0798015
CACTTAA59550.05.0629961
Produced by FastQC (version 0.11.5)