FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16687916
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA36450.050.1662036
CTAGCTG20850.043.3268741
TGGACTA100600.043.0088658
GTGGACT127500.034.1033177
GACCTAT82550.031.8306567
CCTATGG86250.029.9649099
GACCTAC83000.029.4029877
GGACTAC148800.029.173739
TGACCTA65950.028.8180226
ACCTATG96800.026.6993478
ACTACGC84750.024.379448
ACCTACG104100.023.7897178
ACAGTGG76100.023.4543381
AGTGGAC81800.022.9008583
AGACCTA63800.021.3263326
ATGTGAC81300.020.6489093
CAGTGGA94900.018.9884362
TGTGACC85850.018.4483454
CAGACTA42350.018.1888565
CCTACGG139700.017.8302469
Produced by FastQC (version 0.11.5)