FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16687916
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACTA105900.052.752628
GGACCTA35000.048.749666
GTGGACT133400.042.2566037
CTAGCTG18000.038.5617521
GGACTAC161700.034.904849
TTAGGCG35400.034.261821
GACCTAT76650.027.4258067
CCTATGG78650.027.1854979
GACCTAC83950.024.269147
ACCTATG87850.024.0106628
TGACCTA60100.022.7599056
ACCTACG102550.020.498668
GAGGACC108600.018.4286949
TGAGGAC116550.017.7275168
AGACCTA60850.017.391946
CCCTAGG26350.016.72044285-89
CCTAGGG26450.016.65722885-89
CGTGGAC228500.016.3835666
GTGAGGA122200.016.3780547
GCTACGT55800.016.128712
Produced by FastQC (version 0.11.5)