FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14317547
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT321320.22442391842680873Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATG36750.035.2606058
GATCGGA149300.032.493581
TCGGAAG161600.030.0398673
AGTGAGT36100.029.9532431
GTGAGTA40850.026.8343642
ATCGGAA186350.026.3159662
CCTTTAG51750.026.1555984
AGAGCGT202100.024.3292688
AAGAGCG219800.022.3700877
TAACCCC47650.022.056159
GAGCGTC240000.020.7855639
GGAAGAG240550.020.3805735
GAAGAGC260700.019.136696
CTTTAGC73000.019.0330815
TTAGCAT71400.018.6529777
CGGAAGA273450.018.0618954
TTTAGCA94700.014.8998116
ATAACCC71100.014.8840928
TGAGTAT81400.013.537753
GTATAAC87000.013.3223626
Produced by FastQC (version 0.11.5)