FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14372955
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAG27250.09.5310846
CTAAGAG34050.08.8989448
GATCGGA171500.08.1444111
TACACTA43150.07.85565664
AGAGCGT174950.07.7938888
ACACTAA38800.07.2509565
AAGAGCG204500.06.87935267
TCGGAAG208550.06.83811573
ATCGGAA227600.06.45283272
GAGCGTC214400.06.4270989
TAAGAGC57350.06.164099
ACTAAGA44550.06.15378337
GTACACT52500.05.7619763
GTGTACA65950.05.46199751
GAAGAGC265750.05.429546
TGTACAC68000.05.293632
GGAAGAG283800.05.28704935
CACTATA63200.05.25106149
AACACTA56850.05.20307067
GTGCACT57950.05.018213330-34
Produced by FastQC (version 0.11.5)