FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14817346
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAA29850.07.5261374115-119
ATATACC134750.07.42613552
AGGGCCC33650.06.930983520-24
TGGGCCC32550.06.9027534120-124
TATACCC65500.06.81439352
CGCGCAA137250.06.6604078
TATACCG151100.06.52636583
TACCGCG141200.06.1682445
CTCTTAG33404.5110937E-106.0342385
GCGCAAT165550.05.95665269
CCCTAAT40350.05.924564115-119
TATATAC160950.05.7442831
TTAGTCC35802.4501787E-95.6296898
GCCCCTA40350.05.424902115-119
CTTAGTC35055.088259E-85.3394287
ACCCATC81600.05.29263545
TACCCAT78950.05.28799634
TAGTCCC37053.1830496E-85.24549449
CTCCCTA27801.7178761E-55.1784584
Produced by FastQC (version 0.11.5)