FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14817346
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAA30150.08.596068115-119
TACCGCG130700.07.7643495
ATATACC137250.07.4685052
CGCGCAA139450.07.1757578
TATACCC66000.06.99992942
AGGGCCC36750.06.934296620-24
TATACCG156600.06.61846263
TGGGCCC35400.06.3033943120-124
TATATAC169150.06.1066711
GCGCAAT162100.05.99545579
GCCCCTA43750.05.891008115-119
CCCTAAT42250.05.8275237115-119
ATACCGC202300.05.7992954
CTCCCTA28206.5670974E-75.6150944
ACCCATC82350.05.59342585
TACCCAT85800.05.53656154
TTGGCCC50950.05.0860176105-109
ACCGCGC209700.05.08062466
Produced by FastQC (version 0.11.5)