FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14997334
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACTA61650.011.6810752
ATTTGAG95800.011.6691111
ACTATGG61450.011.4796955
ATTTACT72500.010.5818871
CTATGGG69950.010.4963586
TTACTAT70650.010.29497053
TACTATG80800.09.0867934
TTGAGGG126150.08.96248153
TATGGGC93900.08.1258077
TGAGGGC146450.07.7167094
GCAAGAC154850.07.29835139
TTTGAGG176600.06.44291352
ATGGGCC130300.06.13217978
ATTAGTA41550.05.78768365-69
GAGGGCA212500.05.6908425
AGGGCAA211900.05.5371066
GAGCACA118950.05.5069779
GATTAGT44100.05.322409665-69
GATCGGA125150.05.31278661
TGAGTTA46550.05.1031265140-144
Produced by FastQC (version 0.11.5)