FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15488096
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGAGTAAATCTCGTAT171470.11071083237087374TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA256950.09.9465819
AGAGCAC265750.09.5359398
CCCTAAC22902.1827873E-117.85956676
GATCGGA332150.07.63821841
TCGGAAG353700.07.41478353
AAGAGCA382200.07.063747
CGAGTAA79150.06.74942435-39
GGAAGAG392100.06.7385015
ATCGGAA408550.06.52512172
GAAGAGC401950.06.4838156
CGGAAGA451550.05.94967754
GCGAGTA100650.05.73794230-34
CTAACAC31402.634988E-85.73197758
CACACTA25203.0658211E-65.713788
GCCACTA43801.2732926E-115.5885476
AGTCACG102000.05.520517325-29
CTCGTAT105500.05.513825440-44
CGCGAGT102200.05.3831730-34
ACGCGAG105400.05.370040430-34
CAGTCAC115100.05.004812725-29
Produced by FastQC (version 0.11.5)