FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15488096
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTTGATGGTGTAGATCT179550.11592774218341623Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT315350.08.2417018
GATCGGA328900.07.7282161
GAGCGTC349150.07.56757169
AAGAGCG354400.07.25232127
TCGGAAG362350.07.21239473
TAGATCT80150.06.808499340-44
CTAACAC26002.8940121E-96.64569478
GAAGAGC404950.06.63147166
CCCTAAC23159.802716E-86.5308666
GGAAGAG401650.06.4887635
ATCGGAA414100.06.32844832
CGGAAGA456450.05.6939694
CATTACC129200.05.57321641
CACACTA26604.55291E-55.1425028
AGATCTC108150.05.113470645-49
AGTGTAC110250.05.08035425-29
Produced by FastQC (version 0.11.5)