FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15355172
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG44650.09.857531
GATCGGA172050.09.7295561
GAGTGAT54500.09.2475553
TGAGTGA52350.09.0773332
TCGGAAG199600.08.3325283
ATCGGAA233050.07.75453332
AGAGCGT215950.07.7349978
AAGAGCG247200.06.78629647
AGTGATG84650.06.37910374
GAGCGTC288450.06.11534459
TGTGTAG58600.05.922095335-39
TAGATCT62850.05.68200740-44
ACTGTGT64250.05.490978730-34
GGAAGAG315450.05.318035
Produced by FastQC (version 0.11.5)