FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14595270
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA137550.011.3570949
AGAGCAC144000.010.6988158
GCTTAGC26050.09.3962736
GATCGGA192050.08.6099271
TGCTTAG29250.08.1221785
CTTAGCT34900.07.2198317
TCGGAAG232400.07.0631193
GACTCGG42900.06.71275043
AAGAGCA240250.06.65233337
GAAGAGC254900.06.49594646
ATCGGAA277050.06.1165842
CTAGTCC12450.05.666605595-99
AGGCTAG12550.05.506729695-99
GGAAGAG323950.05.33356575
ACTCGGT55450.05.32316264
GGACTAG12400.05.22617730-34
GAGCTAG12550.05.163203270-74
GGCTAGT14050.05.123773695-99
GACTAGC12650.05.12289330-34
GCTAGTC14750.05.07583695-99
Produced by FastQC (version 0.11.5)