FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14818695
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATG38250.013.73949058
AGTGAGT33850.012.5186071
GTGAGTA37800.011.0654562
GTCTCTT67500.09.2002961
TAACCCC54650.08.0348249
TTAGCAT77900.07.6704327
CTTTAGC100700.06.57714755
TCTTTAG107950.06.13614564
TTTAGCA105850.05.9170826
GAGTATA83100.05.28517444
GTATAAC87700.05.25363166
CTCTTTA113800.05.19009263
TGAGTAT84350.05.1235343
ATAACCC81700.05.1107568
Produced by FastQC (version 0.11.5)