FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14192882
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCT41350.023.15454720-24
CTAGCTA42950.022.3590220-24
GGTCCTA51300.018.66354435-39
GCTAACT53600.017.99943525-29
AGCTAAC54150.017.84079620-24
CCTAAGG53550.017.69104640-44
CTAACTA55000.017.5674525-29
CCCTAGC13100.017.36504620-24
GTCCTAA55850.017.14305735-39
CTAAGGT62000.015.67469740-44
TCCTAGC16100.015.5601420-24
TCCTAAG61800.015.51585135-39
ACCTAGC18050.015.4744320-24
TAGCTAA63700.015.30168920-24
GCCTAGC15100.014.20690520-24
CCAGTGA75300.013.04077450-54
TAGCGAA77100.012.81029945-49
GTAGCGA84100.011.81252245-49
CAGTGAT88800.011.15566855-59
CTATAAC95550.010.63829730-34
Produced by FastQC (version 0.11.5)