FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14192882
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGT118600.039.2837222
CCAGTCG169800.027.7001551
AGTCGTA91050.024.6733343
AGTCGTG68750.024.1931513
GTCGTAT116050.012.0987674
CACACTA20200.010.3372356
GTCGTGT98200.010.1185734
AGTCGTC103750.08.7445133
ACTATAG29950.08.1741869
ACACTAT39653.6379788E-125.9927627
ACACACT39201.1459633E-105.69400845
GTCGTCT109500.05.6550444
CACTATA44702.1827873E-115.4770228
TCGTGTT94500.05.25726375
Produced by FastQC (version 0.11.5)