FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17139639
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTAT218600.1275406092275339TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA327550.021.3984221
GAGCACA326950.020.8949689
AGAGCAC331000.020.769868
TCGGAAG355300.019.5836433
ATCGGAA396950.017.8554532
CGGAAGA458750.015.4173224
GGAAGAG490250.014.3461415
GAAGAGC509550.014.1983346
AAGAGCA634100.011.7273727
CAGTCAC155000.07.106343725-29
CACGTCT226550.06.14535510-14
CTCGTAT121500.06.11541540-44
ACACGTC232850.06.11511510-14
CCAGTCA195800.06.059419625-29
CTCCAGT218900.06.018514620-24
AGTCACT146100.05.991973425-29
CACACGT237250.05.959225710-14
ACTCCAG232850.05.942389520-24
ACGTCTG248750.05.79966815-19
CGTCTGA253950.05.765955415-19
Produced by FastQC (version 0.11.5)