FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14501332
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA115500.016.0178979
AGAGCAC122000.015.04647358
GATCGGA141400.013.3470251
ATCTAAG33700.011.9788862
TCGGAAG171000.011.2551793
AAGAGCA192900.010.0759487
CTAAGTA38350.09.9606534
TCTAAGT42650.09.4646873
ATCGGAA202500.09.3268392
GGAAGAG240300.08.4481225
GAAGAGC246100.08.0441736
CATCTAA56200.07.20518451
CGGAAGA280600.06.83236844
TAAGTAC67650.06.4912285
CTACTCC90800.06.37085151
AGTACAC65750.06.1312167
AGTCACA65800.05.9526525-29
GTCACAC69050.05.735038325-29
TACTCCA112550.05.63631732
TACACGC81750.05.63568359
Produced by FastQC (version 0.11.5)