FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14501332
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA145300.012.7165381
ATCTAAG34400.012.3627662
AGAGCGT168400.010.982088
CTAAGTA39750.010.1481764
TCGGAAG184850.010.09872153
TCTAAGT43200.09.8436083
AAGAGCG204800.09.2415187
ATCGGAA207950.09.1163052
GAGCGTC207650.09.1141779
CTACTCC92600.08.6413671
GAAGAGC247150.08.3858376
CATCTAA53900.08.3676531
GGAAGAG252850.08.3106395
ACTCCAA99400.07.9036223
TACTCCA110250.07.38791562
CTCCAAT106500.07.30484964
AGTACAC65200.06.95361337
CGGAAGA293200.06.80538854
TAGATCT60650.06.646882540-44
TCCAATT113600.06.58824255
Produced by FastQC (version 0.11.5)