FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13114040
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA20250.017.071553
CGTCTAA28700.014.5546873
CTACTAA32650.011.2497733
TATACTA52950.010.3376292
CTATACT51900.010.3350191
ATACTAA57700.09.73588853
TAGAGTA42750.09.0976872
CTCAGAT60700.08.4784531
GTTCCTA45350.08.0996492
GTAGAGT60650.07.4098231
ACGACTA59500.07.3839072
TACTAAG28307.2759576E-127.1230514
AGAGTAA63200.06.60948563
TCGTCTA70350.06.4498522
GCTACTA52200.06.34688572
CACGTAA40000.06.3018033
TACTAAA68700.06.28765874
CCCGATA79150.06.09673362
AGTGGTA55650.06.08283472
TTCCTAA62800.06.07817173
Produced by FastQC (version 0.11.5)