FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17518011
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAG41850.08.9441236
CTCAGTG37850.08.37416654
CCTCAGT43500.07.7833373
AGTGTCT49500.07.12556847
GTGTCTC47550.06.81242568
CTGTAGC61100.06.3618177
TGTCTCA53400.05.7964669
GTAGCAT66300.05.64580549
CAGTGTC66500.05.4122556
TCAGTGT66150.05.33208855
GCCTCAG74000.05.06220872
Produced by FastQC (version 0.11.5)