FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14642600
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA20650.017.089853
CGACTAA16500.014.84079553
TAGAGTA32200.010.2892092
CTACTAA30250.09.0473313
TATACTA47150.08.7070942
GTAGAGT44650.08.2792821
ATACTAA48300.07.90298183
AGAGTAA48550.07.86228663
GACTAAT20401.0695658E-97.7640294
CTCAGAT59250.07.70719151
CTATACT52350.07.19996831
GTCTAAG15902.3124489E-46.339074
TACTAAC22051.9414929E-66.2035414
GTCTAAA26954.2069587E-86.14417654
GCTACTA53450.06.06382
TCGTCTA68700.05.870992
ACTAACT22351.5889804E-55.79819155
ACTAACA19901.09874236E-45.78847935
GTCTAAT21605.857251E-55.6661734
TACTAAA58050.05.0848284
Produced by FastQC (version 0.11.5)