FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14642600
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA21350.016.5246183
CGACTAA16300.014.5767123
CTACTAA33500.09.2418073
TAGAGTA33850.08.5116842
ATACTAA48050.08.39127353
CTCAGAT53700.08.237381
CTATACT54050.08.0498751
TATACTA52450.07.96520042
GTAGAGT45800.07.12493041
AGAGTAA51150.06.756613
GTTCCTA41550.06.4142362
AACACTA34752.910383E-116.2157537
CACGTAA38951.8189894E-126.10013873
TCGTCTA66200.06.0931832
TACTAAA56050.05.909024
CACTATA42653.274181E-115.57066979
GTCTAAA29251.3165172E-65.41539344
ACACTAT40601.6625563E-95.32013378
CGAGAAT107550.05.25917671
AACATAA94000.05.20851953
Produced by FastQC (version 0.11.5)