FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15670137
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA95600.019.2563421
TCGGAAG152750.012.4932443
AGAGCAC179700.010.3749228
ATCGGAA195600.010.0508872
GAGCACA202200.09.5764459
CGGAAGA248150.07.9189514
AGTCACA58300.06.593615525-29
GGAAGAG310350.06.3783335
AAGAGCA325850.06.14120057
CAGTCAC70300.05.69338325-29
GAAGAGC358200.05.56647736
CACACGT77750.05.46256910-14
ACACGTC76950.05.332263510-14
CACGTCT81050.05.16910510-14
Produced by FastQC (version 0.11.5)