FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18343352
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTTGATGGTGTAGATCT270230.14731767672560608Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT345750.012.6970198
GATCGGA354800.012.4509131
TCGGAAG358500.012.3897553
GAGCGTC394650.011.2329969
AAGAGCG393050.011.2249567
ATCGGAA403550.011.1006412
TAGATCT100000.09.38681940-44
GAAGAGC485400.09.3415966
GGAAGAG480950.09.3382385
CGGAAGA487950.08.9995584
AGTGTAC117200.07.566884525-29
GAGTGTA132600.06.85093525-29
GTGTACT138350.06.534983625-29
GTAGATC147850.06.34888140-44
AGATCTC153050.06.34029945-49
CGTATCA154700.06.327010660-64
GTGTAGA155550.06.265987440-44
AGAGTGT152550.06.14373925-29
TCATTAA159800.05.981933665-69
TACTTGA161150.05.80936630-34
Produced by FastQC (version 0.11.5)