FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10940006
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC5800.09.67801390-94
CTAGTCC6400.09.22047990-94
GGCTAGT5750.08.26029790-94
GGTACGA64250.06.8343841
GTGTAGA54650.06.71773241
GGTACAG71500.06.44342231
TCGCTAG5252.4760084E-86.3054795140-144
AGGCTAG6858.403731E-105.88324785-89
GTCAAAT74750.05.874371
GTGTAGG51550.05.86493641
GGTATGG79300.05.8096431
GGGGGGG20151.3173494E-45.7159391
GGTCCGG64250.05.6019541
GTCTCAG27302.138515E-65.5373161
GGGTCTA17000.00352078355.5047431
GGTACCT23553.6755482E-55.50204661
GAGCTAG6556.6407665E-85.493499360-64
GTTACGA65800.05.4699931
GTTACGG88800.05.4313091
GTCTACA56300.05.3701151
Produced by FastQC (version 0.11.5)