FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10940006
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG10300.00571622376.99009751
GTTACAG45650.06.6241321
GGTACGA66200.06.30798241
GGTACAC46650.06.01912641
GGTACGG109150.06.0025821
GGCTAAG20803.2925323E-55.88445231
GTCAAAG119200.05.79849771
GGTCCAA17000.00351333465.50572971
GTGTCAA56600.05.46981331
GACCTAG4754.1946332E-55.45669185-89
GTCTGAG31852.1243795E-75.4252821
GGTACGT41354.7293724E-105.3976741
GTATAGA57900.05.34700251
GGTACAT60750.05.33318571
CCCTAAA27101.0976322E-55.31350561
GTCTACA55750.05.29492041
GGTCCGA42701.1041266E-95.22702171
GTGTAAC26454.1431987E-55.171881
GTTACTG33857.4706804E-75.1047331
GGTCCAG52852.0008883E-115.04054161
Produced by FastQC (version 0.11.5)