FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18700375
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGCATGAACGTAATGCTCATAACTTCCCTCTAGACCTAGCTGCTGT1831750.9795258116481621No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1048230.5605395613724324Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCATG271250.0112.046464
TATGCAT273250.0111.200313
GTTATGC288250.0106.181091
TTATGCA299750.0101.466312
TGAACGT341800.088.666779
TGCATGA358650.084.641325
ATGAACG416700.073.07548
CATGAAC430000.070.965837
GCATGAA430450.070.524666
ACCTAGC233800.026.36188335-39
GACCTAG233400.026.30836735-39
GATCGGA613800.026.0390421
TCGGAAG630450.025.7938633
TCTAGAC241150.025.78493730-34
CTAGACC238100.025.69801930-34
CTCTAGA239300.025.58118830-34
CCTAGCT237750.025.5060735-39
CCTCTAG239100.025.27743530-34
ATCGGAA652750.025.0672592
CTAGCTG240150.025.01756140-44
Produced by FastQC (version 0.11.5)