FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12453159
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA112900.018.5168511
AGAGCGT148900.014.3116248
TCGGAAG164000.013.3942633
ATCGGAA185700.012.1779822
AAGAGCG183000.011.88085657
GAGCGTC179850.011.8087379
GAAGAGC197050.010.8510536
GGAAGAG219150.010.282535
TAGATCT47500.09.30562940-44
CGGAAGA275800.08.1735914
GTAGATC63950.07.047007640-44
AGAGTGT68600.06.653477725-29
GTAGGGA66600.06.6169915-19
AGTGTCA67000.06.57597525-29
GAGTGTC68900.06.41553225-29
AGATCTC71550.06.278355645-49
TGTAGGG72950.06.119975615-19
TAGGGAA80200.05.818136715-19
GTGTAGA80850.05.805485740-44
TCGTGTA86350.05.60404210-14
Produced by FastQC (version 0.11.5)