FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21369345
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAG72950.037.69796
CTACACA104900.033.559744
CTGTAGC101950.026.833267
CACATAC136350.025.9768837
TACACAT139950.025.617435
ACACATA141950.025.1043436
TATAGTC148950.024.9882164
GTAGCAT118650.023.7844629
GCCTGTA122250.023.0842785
CCTACAC132000.022.8606663
TCCTACA136500.022.2653052
ACATACT168700.020.9527688
TGTAGCA136450.020.6817658
TAGGACC27650.020.0481666
CTCCTAC159500.019.3678051
ATAGTCA203700.018.4483955
GACCCCC97100.017.9420139
TAGTCAT212800.017.8962966
TATATAG216800.017.8719442
CATACTT210850.017.071499
Produced by FastQC (version 0.11.5)