FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14609294
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA68750.025.445939
GCCACTC68000.025.1972318
AAGCCAC105200.016.4240516
AGCCACT113250.015.2566017
ACAAAGC159700.011.2720943
GGCCTAA34400.010.59174575-79
TACAAAG184500.010.0311532
ATACAAA205600.09.1241491
CTCACTA43050.08.62922415-19
AAAGCCA220650.08.4178385
CAAAGCC265800.07.25909384
TAGCTCT58150.06.784766740-44
ACTCACT55100.06.68980615-19
CCCTTAA90150.06.62849574
TACACAC57850.06.54615850-54
TAGCCAC59650.06.517501425-29
GGGCCCC63300.06.18704110-114
CTACACA59850.06.062761350-54
GGGGCCC62300.06.032123110-114
ACACACT63550.06.026985650-54
Produced by FastQC (version 0.11.5)