FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14609294
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACTC66000.024.3234468
CCACTCA71150.022.562869
AGCCACT107150.015.5869097
AAGCCAC106550.015.53955656
ACAAAGC156750.010.6088583
GGCCTAA33100.010.09226675-79
TACAAAG187000.08.9355282
ATACAAA207250.08.39757251
AAAGCCA212700.08.1566865
CTCACTA42750.07.91472415-19
TAGCCAC54450.06.71644625-29
ACTCACT54250.06.5819715-19
CAAAGCC262200.06.5344424
CCCTTAA88750.06.40800674
TACACAC58800.06.1215750-54
GGGCCCC60100.06.061151110-114
CTACACA59400.06.059736350-54
ACACACT62200.05.97213350-54
TAGCTCT60850.05.963423740-44
GCCCCTA61750.05.9458275110-114
Produced by FastQC (version 0.11.5)