FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14433506
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT67900.015.5869598
CACTATA71600.014.4789729
AACACTA72600.014.2803797
AAACACT106350.09.7485236
TAGCCCT73500.09.7046042
GCCCTTA67250.09.3146474
AGAGCAC100350.09.1834628
CAAACAC119900.08.9470715
GATCGGA105600.08.8543761
GAGCACA112250.08.1452739
TATTTAG82300.08.135269
ATCAAAC138300.07.9133583
GTAGCCC91350.07.45849941
TCAAACA158150.06.965654
CCCCCTA26505.275069E-106.8379561
CCTTATT114850.06.83296736
CCCTTAT104400.06.7583315
TCGGAAG158300.06.36775833
AGCCCTT122200.06.06883673
ATCGGAA183500.05.7325352
Produced by FastQC (version 0.11.5)