FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14433506
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT70850.015.9520238
AACACTA73250.015.8229597
CACTATA74400.015.2891649
AAACACT105850.010.8137366
TAGCCCT74150.010.10395052
GCCCTTA67200.09.9628754
CAAACAC118200.09.8056835
ATCAAAC139300.08.4757913
GATCGGA110000.08.2624681
TATTTAG88250.07.91328059
TCAAACA156350.07.64330434
GTAGCCC93650.07.5310661
CCCTTAT102500.06.7424365
AGAGCGT149750.06.1531728
AGCCCTT119700.06.13470273
CCTTATT116800.06.04021936
TCGGAAG164050.05.9244093
ATCGGAA185300.05.59829852
TATCAAA235050.05.42477852
GAGCGTC177400.05.39756169
Produced by FastQC (version 0.11.5)