FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16394750
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT452020.2757102121105842TruSeq Adapter, Index 5 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT39700.038.4190861
GTGAGTA45250.033.5737722
TAATGGG55500.027.499679
GAGCACA279600.025.8512239
GATCGGA287800.025.3177361
AGAGCAC299650.024.2656368
TCGGAAG302600.024.1498723
ATCGGAA332550.022.2356432
AAGAGCA397500.018.708887
GGAAGAG420500.018.2163015
CGGAAGA407200.018.1390654
GAAGAGC430050.017.4602286
TGAGTAT106250.014.8399663
GAGTATA109750.014.3653074
AGTCACA118650.012.2077725-29
CTCGTAT126050.011.56004840-44
CACATTA68700.011.2127376
TCACACA128450.011.1084630-34
GTCACAC132400.011.00521825-29
CAGTCAC136900.010.81174525-29
Produced by FastQC (version 0.11.5)