FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16394750
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCGTAGCGTGTAGATCT469100.2861281812775431Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT38650.032.331061
GTGAGTA43800.028.7670612
GATCGGA292800.024.8869021
TCGGAAG311100.023.4887853
TAATGGG55850.022.8162679
ATCGGAA336550.021.885562
AGAGCGT334250.021.5819558
AAGAGCG355900.020.4508977
GAGCGTC380500.019.1100239
CGGAAGA409650.018.066694
GGAAGAG426800.017.52595
GAAGAGC433250.017.2649846
TAGCGTG92150.015.59545735-39
TAGCGTA107750.013.51010130-34
TAGATCT109900.013.46910440-44
GAGTATA113100.011.839924
TGAGTAT113050.011.7813663
GAGTGTA127500.011.50802225-29
AGCGTAG132850.011.16350130-34
AGTGTAG134350.011.08203625-29
Produced by FastQC (version 0.11.5)