FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13824885
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTAT301030.2177450300671579TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA176150.030.3689569
AGAGCAC189250.028.380938
TCGGAAG213100.025.3841383
GATCGGA217100.025.1488691
ATCGGAA235850.023.3327832
AAGAGCA244550.022.0220457
GGAAGAG279200.019.5984545
CGGAAGA305950.017.9085084
GAAGAGC343300.016.0649246
AGTCACA78000.012.55345525-29
ACACGTA77200.012.16156930-34
CAGTCAC89550.011.51320925-29
CCAGTCA100400.010.82834825-29
GTCACAC92750.010.43288225-29
CACGTAT94350.09.98148530-34
TCACACG97650.09.61467630-34
ACTCCAG123700.09.00990620-24
CTCGTAT102450.08.95317540-44
CACGTCT125550.08.739531510-14
ACGTATC112050.08.57182530-34
Produced by FastQC (version 0.11.5)