FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13824885
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCTGATAGTGTAGATCT325080.23514119647288206Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG214250.023.814243
GATCGGA216700.023.7920861
ATCGGAA237400.021.4925462
AGAGCGT243850.021.0710018
AAGAGCG274150.018.6109817
GGAAGAG278150.018.4727045
CGGAAGA310800.016.647894
GAAGAGC349050.014.9678816
GAGCGTC349600.014.717859
TAGTGTA73150.013.89109135-39
AGTGTAG75150.013.57892840-44
ATAGTGT83550.012.23088635-39
TAGATCT90300.011.69920940-44
ACACTCT38300.011.0925571
GAGTGTT88850.010.99913125-29
GATAGTG94400.010.93183735-39
AGAGTGT93050.010.719213525-29
AGTGTTG92550.010.69936625-29
TAGGGAA109300.09.44155315-19
TGATAGT110650.09.41744735-39
Produced by FastQC (version 0.11.5)