FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14616354
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG69150.070.323791
GAGTGAT78500.061.4553873
TGAGTGA78400.060.8973462
AGTGATG103800.047.0303544
ATGGTTG111000.043.525228
TGGTTGA129950.037.289349
GATGGTT130050.037.259017
TGATGGT221700.022.3097826
GTGATGG230950.021.7285615
TAGTGTG54550.017.86743515-19
GTAGTGT62050.015.75419915-19
AGTGTGG89000.011.40426815-19
TGAGGTA102200.010.28530810-14
GGTAGTG101200.09.90141115-19
AGGTAGT117050.08.92963115-19
GGTTGAG122250.08.59843310-14
GAGGTAG138850.07.912686310-14
TTGAGGT138150.07.702626710-14
GTTGAGG138550.07.62842410-14
GTGTGGA152800.06.83109220-24
Produced by FastQC (version 0.11.5)