FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18211837
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT45550.019.8059161
GTGAGTA55250.016.9463672
TAGCATG52400.013.8758278
TAACCCC74750.012.2309149
CCACTCA61350.08.4485919
GTCTCTT90150.08.2987491
ATAACCC126700.07.21598868
TGAGTAT147350.06.6444453
GCCACTC80050.06.47500238
GAGTATA156150.06.13168144
TATAACC167900.06.08837847
GGCCTAA20000.05.97526475-79
GTATAAC168150.05.90807776
AGCCACT101400.05.46659377
CGCCTAA40900.05.24536945-49
AGCATGA179950.05.24065839
AAGCCAC115000.05.07050476
Produced by FastQC (version 0.11.5)