FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16823755
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA30350.0101.129061
CTAGAAC35500.086.394763
TCTAGAA39550.077.597572
TAGAACC81150.038.4154174
ACCGGTC161600.019.2908528
CCGGTCC168850.018.5905769
AACCGGT191000.016.3214747
AGAACCG200900.016.1622855
TCTTGTG114800.014.3092881
GACTCAC38950.012.5784321
CTAGGTC17000.012.451195135-139
AGGCTAG18500.012.22024275-79
GCTAGGT18000.012.159444135-139
GAGCTAG17900.012.14686550-54
GCTAGCT18500.011.519355130-134
CTAGTCC20700.011.47798175-79
GGCTAGT20600.011.39389775-79
CTTGTGG145050.011.3737262
GAACCGG287550.011.2418836
TCGCTAG19250.011.070549130-134
Produced by FastQC (version 0.11.5)