FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14296201
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG40850.012.8743841
CCACTCA47050.011.16953859
TGAGTGA49850.010.1112922
GAGTGAT61300.09.1623723
GCCACTC67250.08.2427188
TCCCTAA37400.07.12180334
AGTGATG85950.06.5329384
TAGTGTG21600.06.266012715-19
AGCCACT86650.06.23109967
ATGGTTG101400.05.8216748
AAGCCAC98000.05.7298146
TACAAAG125600.05.67569832
GGCCTAA21900.05.45767575-79
CCCTAAA56750.05.3276875
GATGGTT116300.05.199627
CTAAATT68500.05.14964777
TGGTTGA108750.05.0310239
Produced by FastQC (version 0.11.5)