FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14507835
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG33950.016.7642751
GAGTGAT51200.012.3761063
TGAGTGA46800.012.0010732
AGTGATG73600.08.5093134
CTAGCGT6450.08.48380790-94
ATGGTTG95100.06.73707778
TAGTGTG21900.06.57470215-19
TGGTTGA108650.05.9631389
GCTAGCG9000.05.92056285-89
GATGGTT107800.05.87659747
TGCTAGC10355.4569682E-115.00917185-89
Produced by FastQC (version 0.11.5)