FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14507835
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG54150.053.4486121
GAGTGAT63050.046.481143
TGAGTGA63000.045.608862
AGTGATG86000.034.4953274
ATGGTTG112550.027.0617778
GATGGTT122700.025.2329147
TGGTTGA126350.023.707579
GTGATGG198050.015.8867525
TAGTGTG39750.015.75483815-19
TGATGGT206750.014.9743666
GTAGTGT50100.012.58630315-19
AGTGTGG66300.09.85834715-19
GGTAGTG71450.09.127624515-19
TGAGGTA88200.07.62453610-14
AGGTAGT94500.06.977429415-19
GAGGTAG116750.06.105389610-14
GGTTGAG112500.06.00323610-14
CTAGCGT7707.094059E-115.79781790-94
GTGTGGA120700.05.630028220-24
GTTGAGG123850.05.60423210-14
Produced by FastQC (version 0.11.5)