FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15831874
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA36650.023.3778367
ACACACT47900.018.4877096
AACACAC65350.013.3306675
ACACTAT68300.013.2825418
CACTATA72450.012.819849
CAACACA74650.012.2488984
CCGTTAA90850.09.2722264
ACAACAC109700.08.9915723
CGTTAAA108700.07.74940635
TCCGTTA115700.07.65408853
ATACAAC127500.07.60629221
ATCTAGC24950.07.15651325-29
GTTAAAT120050.06.83685166
TACAACA170950.06.31757932
CTAGCTA28450.06.22548525-29
GTTCCGT161850.05.5448191
Produced by FastQC (version 0.11.5)