FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13739736
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG79800.017.0625252
AACACTA44600.013.8815557
ACACTAT48050.012.7350328
CACTATA56350.011.3707629
GGGGGGT30850.010.2705145
ATATGGG143050.09.29098930-34
GGGGGGA51700.08.496385
TTATATG167450.08.0747430-34
TATATGG170600.07.9931730-34
TCTTATA169050.07.83624725-29
TATGGGG87650.07.68850830-34
CTTATAT178350.07.50050930-34
AAACACT92250.07.25756746
GGGGGGC47200.07.0179535
ACCAAAC93750.06.915283
CAAACAC94950.06.9014615
CAACTAT95400.06.7534161
GCCCTTA50750.06.3872654
ACTATAA94100.06.35370453
TAGCCCT58150.06.19447762
Produced by FastQC (version 0.11.5)