FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005186988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005186988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13739736
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT82100.022.2826614
AGTGGTA95100.019.5373255
AGCAGTG101350.017.9801042
GTGGTAT121800.015.6093266
GCAGTGG123100.014.9202473
TGGTATC144750.013.33363157
AACACTA48200.013.2976597
ACACTAT48500.013.2147328
GGTATCA149350.013.1633828
AAGCAGT141000.013.1270531
GTATCAA159550.012.592669
CACTATA55450.011.2986829
AAACACT85400.08.011126
GCCCTTA54800.07.75439554
AGAGTAC78800.07.673587315-19
ACCAAAC99100.07.55881263
GGGGGGG66250.07.3929632
TAGCCCT54700.07.24220562
CAAACAC99450.07.2413765
CAGAGTA81050.07.05200915-19
Produced by FastQC (version 0.11.5)