FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13987668
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTA43450.014.0882212
TGAGTAG43250.013.8203483
TAGGAGG50750.012.6246547
GAGTAGG48350.012.3611074
AGTGAGT50100.012.1498871
TAGCATG35300.07.54557858
GTAGGAG94150.06.95800926
AGTAGGA105650.06.5413245
AGGAGGG108650.06.1619588
GGAGGGG114350.05.60298359
CTAGGAG24800.05.5733750-54
Produced by FastQC (version 0.11.5)