FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14006724
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA13850.043.3776781
CTAGAAC14350.041.1403433
TCTAGAA19650.030.7984092
TAGAACC49300.013.2880154
CTAGTCC9750.010.19113575-79
GCTAGTC11600.09.43482375-79
GGCTAGT11650.08.89989175-79
GAGCTAG11350.08.75383850-54
CTAGCTA9500.08.639429130-134
CTAGGTC9150.08.340241135-139
GCTAGGT10250.08.147568135-139
AGCTAGG10950.07.8897095135-139
AGGCTAG13350.07.44296575-79
TCGCTAG12150.06.8736196130-134
TAGCTAG10400.06.784012135-139
CCGGTCC109500.06.7063929
CGCTAGC12850.06.3871264130-134
AGCTAGA16150.06.33039950-54
GCTAGCT12100.06.3070216130-134
TCTTGTG86800.06.1709211
Produced by FastQC (version 0.11.5)