FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005187039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005187039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11459842
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGA8050.019.677021
CTAGAAC9150.017.3114763
TCTAGAA13551.8189894E-1211.1586712
AACACTA25503.274181E-117.34117657
CACTATA29801.8735591E-106.523499
ACACTAT28452.9922376E-96.3268898
TAGAACC34201.6705962E-75.2631584
CTAGATC5753.0574884E-55.0086956140-144
Produced by FastQC (version 0.11.5)